Fig. 5.

Cytotoxicity (MTS) of flotillin-1- and flotillin-2-depleted H441 following aSNP treatment with 6–300 μg/ml Sicastar Red (30 nm). uc untransfected control (no siRNA treatment), neg Silencer^® Negative Control #1 siRNA and F12 Silencer^®Select siRNA for flotillin-1 and flotillin-2 in combination (siRNA concentration: 50 nM). Data are depicted as mean ± SD of 3 independent experiments with n = 3 samples for each treatment. For statistical analysis, two-way ANOVA with Bonferroni′s post-test was applied. *P < 0.05, **P < 0.01 and ***P < 0.001 compared to the untreated control of the respective siRNA pre-treatment. Blue asterisks: “one-way ANOVA: within the untransfected control”, red asterisks: “two-way ANOVA: within a concentration of aSNP”. After 4-h exposure and 20-h recovery, subtoxic concentrations of 6 and 60 μg/ml Sicastar Red showed a significantly reduced viability of flotillin-1/2-depleted cells compared to untransfected cells