Table 1.
Water | PBS | RPMI | ||||
---|---|---|---|---|---|---|
Rh/nm | μ2 (90°) | Rh/nm | μ2 (90°) | Rh/nm | μ2 (90°) | |
Sicastar Red, 30 nm | 12.6 | 0.10 | 66.2 | 0.16 | 58.1 | 0.17 |
Sicastar Red, 70 nm | 33.2 | 0.06 | 34.1 | 0.03 | 31.6 | 0.03 |
Sicastar Red, 300 nm | 157 | 0.02 | 151 | 0.01 | 151 | 0.04 |
Hydrodynamic radii (Rh, with μ2 values as an estimate for the polydispersity) in different aqueous solutions (Milli-Q water, phosphate buffered saline (PBS, without Ca2+ and Mg2+) and serum-free cell culture medium RPMI) were obtained by dynamic light scattering (angular-dependent measurements)