Table 1.

Primer Sequences Used in Real-time PCR

Genes	Primers	Length (bp)
Twist1	Forward: 5′-TGTCCGCGTCCCACTAGC-3′ Reverse: 5′-TGTCCATTTTCTCCTTCTCTGGA-3′	63
Snail1	Forward: 5′-TGCAGGACTCTAATCCAAGTTTACC-3′ Reverse: 5′-GTGGGATGGCTGCCAGC-3′	71
E-cad	Forward: 5′- GAACAGCACGTACACAGCCCT-3′ Reverse: 5′-GCAGAAGTGTCCCTGTTCCAG-3′	76
N-cad	Forward: 5′- GACGGTTCGCCATCCAGAC-3′ Reverse: 5′-TCGATTGGTTTGACCACGG-3′	66
MMP-2	Forward: 5′-GACATACATCTTTGCTGGAGAC-3′ Reverse: 5′-TTCAGGTAATAGGCACCCTT-3′	180
MMP-9	Forward: 5′-CTTCACTTTCCTGGGTAAG G-3′ Reverse: 5′-CACTTCTTGTCGCTGTCAAA-3′	105
TIMP-1	Forward: 5′-GGAGAGTGTCTGCGGATACTTC-3′ Reverse: 5′-GCAGGTAGTGATGTGCAAGAGTC-3′	100
TIMP-2	Forward: 5′-ACCCTCTGTGACTTCATCGTGC-3′ Reverse: 5′-GGAGATGTAGCACGGGATCATG-3′	129
MT1-MMP	Forward: 5′-CCTTGGACTGTCAGGAATGAGG-3′ Reverse: 5′-TTCTCCGTGTCCATCCACTGGT-3′	146
RANKL	Forward: 5′-CAGAAGATGGCACTCACTGCA-3′ Reverse: 5′-CACCATCGCTTTCTCTGCTCT-3′	203
OPG	Forward: 5′-GGAACCCCAGAGCGAAATACA-3′ Reverse: 5′-CCTGAAGAATGCCTCCTCACA-3′	225
GAPDH	Forward: 5′-TGCACCACCAACTGCTTAGC-3′ Reverse: 5′-GGCATGGACTGTGGTCATGAG-3′	87