Fig. 5.

Effect of chloramine-T on the LNA-OOH reducing activity of HDL. a Incubation of LNA-OOH with apoA-1, b incubation of LNA-OOH with HDL. The liposomal suspension containing LNA-OOH was prepared using the same procedure described above (LNA-OOH; 0.5 mM, DM-PtdCho; 10 mM, cholesterol; 5 mM). To 100 μL of the liposomal solution, 600 μL of apoA-1 solution or HDL solution was added to adjust the final concentration to 0.5 mg protein/mL. For treatment with chloramine-T, the buffer solution of apoA-1 or HDL (1.0 ml/mL) was mixed with chloramine-T (0.5 mM) and incubated at 37 °C for 1 h. Then the chloramine-T-treated solution was added to the 100-μL liposomal suspension to adjust the final volume to 600 μL (final concentration of apoA-1 and HDL: 0.5 mg/mL). After incubation at 37 °C for 6 h, total lipids were extracted and the extract subjected to quantitative normal-phase TLC analyses as described above.