Table 1. Developmental Classification of Lateral Roots and Lateral Root Primordia.
Genotype | Stage ii to iv | Ratio Mutant:Wild Type | Stage v-vii | ratio Mutant:Wild Type | Emerged | Ratio Mutant:Wild Type | Elongated | Ratio Mutant:Wild Type | n |
---|---|---|---|---|---|---|---|---|---|
The wild type | 13 ± 1 | NA | 3 ± 1 | NA | 4 ± 0.5 | NA | 10 ± 1 | NA | 19 |
glr3.4-1 | 22 ± 2 | 1.7 | 5 ± 1 | 1.7 | 4 ± 1 | 1.0 | 10 ± 1 | 1.0 | 12 |
glr3.4-2 | 23 ± 1 | 1.8 | 3 ± 1 | 1.0 | 4 ± 0.7 | 1.0 | 10 ± 1 | 1.0 | 20 |
rescued | 13 ± 1 | 1.0 | 3 ± 1 | 1.0 | 6 ± 1 | 1.5 | 9 ± 1 | 0.9 | 11 |
glr3.2-1 | 22 ± 1 | 1.7 | 4 ± 1 | 1.3 | 4 ± 0.4 | 1.0 | 9 ± 1 | 0.9 | 14 |
glr3.2-2 | 21 ± 2 | 1.6 | 5 ± 1 | 1.7 | 4 ± 0.6 | 1.0 | 11 ± 1 | 1.1 | 6 |
glr3.3-1 | 17 ± 1 | 1.3 | 3 ± 1 | 1.0 | 5 ± 1 | 1.2 | 11 ± 1 | 1.1 | 6 |
glr3.3-2 | 14 ± 1 | 1.1 | 3 ± 1 | 1.0 | 5 ± 0.4 | 1.2 | 11 ± 1 | 1.1 | 9 |
glr3.2-1 glr2.4-1 | 21 ± 3 | 1.6 | 7 ± 3 | 2.3 | 3 ± 1 | 0.7 | 14 ± 3 | 1.4 | 7 |
glr3.2-1 glr2.4-1 | 20 ± 4 | 1.5 | 7 ± 4 | 2.3 | 3 ± 1 | 0.65 | 13 ± 2 | 1.3 | 5 |
Each root or primordium was classified according to developmental stage (stage ii-iv, stage v-vii, emerged, or elongated) and enumerated for each genotype. The ratio column shows how the different genotypes compare to the wild type for each stage. The “rescued” genotype refers to a glr3.4 mutant transformed with GFP-GLR3.4 controlled by the GLR3.4 promoter. NA, not applicable.