Figure 3.

RGA5 Is Subject to Alternative Splicing and Produces Two Hypothetical Protein Isoforms: RGA5-A and RGA5-B.

(A) Structure of RGA5-A and RGA5-B transcripts. RGA5-A, as described by Okuyama et al. (2011), is produced by splicing of three introns from the primary RGA5 transcript. RGA5-B is produced by splicing of the first two introns and retention of intron 3. E1, exon 1; E2, exon 2; E3, exon 3; E4, exon 4.

(B) RGA5-A and RGA5-B differ only in their C terminus. Intron retention in RGA5-B leads to a divergent C terminus (underlined) and disruption of the RATX1 domain present only in RGA5-A (marked in gray). In the rest of the proteins, including CC, NB, and LRR domains, as well as the first half of C-terminal non-LRR sequences, RGA5-A and RGA5-B are identical.