Table 1. FRET-FLIM Measurements in N. benthamiana Show That AVR-Pia and AVR1-CO39 Interact with RGA5-A and RGA5-B, Respectively.

Donor	Acceptor	τa	se	Δb	nc	FRET %d	P Valuee
AVR1-CO39:CFP	no acceptor	2.837	0.039	–	72	–	–
AVR1-CO39:CFP	YFP:RGA5-A	2.707	0.051	0.130	20	3	0.21
AVR1-CO39:CFP	YFP:RGA5-B	2.223	0.053	0.614	33	21.7	3 × 10−14
AVR-Pia:CFP	no acceptor	2.523	0.041	–	63	–	–
AVR-Pia:CFP	YFP:RGA5-A	2.129	0.042	0.394	29	15.5	1.2 × 10−9
AVR-Pia:CFP	YFP:RGA5-B	2.381	0.040	0.142	20	6	0.015

^aMean lifetime (in nanoseconds). For each nucleus, average fluorescence decay profiles were plotted and fitted with exponential function using a nonlinear square estimation procedure, and the mean lifetime was calculated according to τ = ∑ α_iτ_i²/∑ α_iτ_i with I(t) = ∑α_i e^−t/τi.

^bΔt = τ_D − τ_DA (in nanoseconds).

^cTotal number of measured nuclei.

^dPercentage of FRET efficiency: E = 1− (τ_DA/τ_D).

^eP value of the difference between the donor lifetimes in the presence and absence of acceptor (Student’s t test). –, treated.