Table 1.

The SMPD1 gene was amplified by seven primer pairs. Exon 2 was amplified by two separate primer pairs to assist subsequent sequencing reactions

exon	Primer sequence (5'to 3‘)	length	Tm
1-forward	GAAGCGCGACAATGCCCCGCTA	445 bp	60.5°C
1-reverse	AGATGCCACCCTCTCCATCAGGGA
2A-forward	GTGTGCACTGAGCTTGGTGCACTG	493bp	60.3°C
2A-reverse	GTCTGCACAGTCAGGGTCCGTG
2B-forward	TGCTGCCTGAGTTACAGGGCAA	1058bp	59.4°C
2B-reverse	GCACTAAGGACTCATGCTAGAGCA
3-forward	GGCTAGACTGTGAGCTCCTTGCAG	441bp	59.1°C
3-reverse	CAACAGTGACCATGAGCTGAATCC
4-forward	GTTGAGCTGGAGCACCTCTG	354bp	58.8°C
4-reverse	CCCACTCCATGGGACAACAG
5-forward	GACAGGGTGAGTGTCTGAAGGCTG	490bp	59.4°C
5-reverse	GTACACACGGTAACCTGCAAGGA
6-forward	ACATCGGCCTTAATCCTGGTGAG	678bp	59.6°C
6-reverse	ACCGGATGATCTTGCCTGGTTGAA