Figure 4.

nPOPG inhibits RSV infection of HEp2 cells in tissue culture.

Notes: Monolayers of HEp2 cells were incubated in six well plates for 1 h prior to the addition of RSV either without lipids or with 200 μg of liposome POPG or nPOPG. Serial 10-fold dilutions of RSV from 2 × 10⁴ − 2 × 10² infectious particles per mL were added to duplicate wells in the absence or presence of lipids. After a viral adsorption period of 1 h, the unbound virus and the lipid were removed from each well, and the monolayers were overlaid with an agar-containing medium for the development of plaques. After 6 d, the cultures were fixed with 1% buffered formalin and stained with 0.05% neutral red, and plaques were counted. The data shown are means ± SE from three independent experiments. *indicates P < 0.001.

Abbreviations: POPG, liposomal palmitoyl-oleoyl-phosphatidylglycerol; nPOPG, nanodisc palmitoyl-oleoyl-phosphatidylglycerol; RSV, respiratory syncytial virus; SE, standard errors of the mean.