Figure 6.

nPOPG blocks RSV binding to epithelial cells. (A) Monolayers of HEp2 cells, at 18°C, were challenged with RSV at MOI = 0–10, as indicated, in either the absence or presence of 200 μg/mL nPOPC, nPOPG, or liposome POPG. UN identifies cultures that were not infected with the virus. The cultures were washed with PBS three times and harvested and processed for SDS-PAGE and immunoblotting. GP indicates the location of the viral G protein, and β-actin blotting was used to normalize the recovery of cells from each well. (B) The quantification of three independent immunoblotting experiments is shown.

Note: Values are means ± SE for three independent experiments.

Abbreviations: MOI, multiplicity of infection; nanoPG, nanodisc palmitoyl-oleoyl-phosphatidylglycerol; nanoPC, nanodisc palmitoyl-oleoyl-phosphatidylcholine; UN, unifected control; GP, viral G-glycoprotein; PFU, plaque forming units; RSV, respiratory syncytial virus; SE, standard errors of the mean.