a, Representative comparison of mRNA abundance and translational efficiency after a 3-h treatment with an ATP site inhibitor (PP242) versus an allosteric inhibitor (rapamycin). b–d, Free energy, length and percentage G+C content of the 5′ UTRs of mTOR target versus non-target mRNAs (error bars indicate range, non-target n = 5,022, target n = 144, two-sided Wilcoxon). e, Functional classification of translationally regulated mTOR-responsive mRNAs. f, Chemical structure of INK128. g, Representative western blot from three independent experiments of mTOR-sensitive invasion genes in PC3 cells after a 48-h drug treatment. Rapa, rapamycin.