Skip to main content
. Author manuscript; available in PMC: 2013 Jun 27.
Published in final edited form as: J Am Chem Soc. 2012 Jun 15;134(25):10428–10435. doi: 10.1021/ja3003205

Figure 2.

Figure 2

The mechanical unfolding of AFV3-109. A) Force-extension curves of (GB1)4-AFV3-109-(GB1)4. Dotted lines are WLC fits to the force-extension curves. The unfolding events of the GB1 fingerprint domains are characterized by a ΔLC of ∼18 nm (colored in blue). The mechanical unfolding of AFV3-109 can proceed through multiple pathways: in curve a), the unfolding of AFV3-109 is an apparent two-state unfolding event with a ΔLC of 38 nm; in curve b), the unfolding of AFV3-109 proceeds in a three-state unfolding pathway involving a stable intermediate state, with a ΔLC(N-I) of 12 nm and a ΔLC(I-U) of 27 nm. In some cases, the N-I unfolding event was not observed, and only the I-U unfolding was observed (curve c). For clarity, the two-state unfolding event is colored in orange, the N-I event is colored in red and the I-U event is colored in green. B) ΔLC histograms for mechanical unfolding events of AFV3-109. Gaussian fits to the experimental data shows a ΔLc of 12.0 ± 1.2nm (n=298), 27.3 ± 1.3nm (n=790), and 37.9 ± 1.1nm (n=521).