FIGURE 2.

Antibiotics regulate selenoprotein activities. EMT6 cells carrying recombinant TR1 or GPx1 were grown in the absence or presence of Dox (10 μg/ml), CP (25 μg/ml), or G418 (100 μg/ml), TR1 and GPx1 were purified, and their activities were measured spectrophotometrically. Specific activities are expressed as the percentage of that found in cells grown in the absence of antibiotics (measured specific activities in control cells were: TR1, 409.6 ± 57.2 units; and GPx1, 460.2 ± 18.1 units). Values are the means ± S.D. of three independent experiments. *, p < 0.01. Experimental details are given under ”Experimental Procedures.“