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. 2013 Apr 15;288(21):14716–14726. doi: 10.1074/jbc.M112.448258

FIGURE 5.

FIGURE 5.

An antibody that preferentially recognizes NEDD8 Q40E. A, 2-fold serial dilutions of purified recombinant NEDD8 Q40E or NEDD8 (3.125–200 ng) were analyzed by immunoblot analysis with the indicated antibodies. Graphs of quantitative analyses comparing signal intensity detected using a Licor Odyssey are shown for each (n = 3). Error bars represent S.E. B, yeast lysates from a rub1Δ yeast strain (MPY156) expressing vector control, NEDD8, or NEDD8 Q40E were immunoblotted with the NEDD8 or NEDD8 Q40E antibodies.