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. 2013 Apr 15;288(21):14716–14726. doi: 10.1074/jbc.M112.448258

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Deamidated NEDD8 is removed more efficiently by the CSN than NEDD8. A, haploid rub1Δ yeast strains expressing NEDD8 (MPY174) and galactose-inducible CIF (WT) or catalytically inactive CIF (C109S) were grown to late log phase and induced with galactose. Cell lysates prepared at the times after galactose addition were analyzed by immunoblotting with the indicated antibodies. B, an affinity-purified CSN was added to lysates from rub1Δ rri1Δ strains (MPY201) expressing NEDD8 or NEDD8 Q40E. Samples were analyzed at the indicated times by immunoblotting for yeast CUL1.