FIGURE 6.

Influence of alterations of the stalk region on IL-6 dependent signal transduction. A, equal numbers of Ba/F3-gp130 cells were cultured for 2 days with increasing amounts of Hyper-IL-6 (0- 10² ng/ml) or human IL-6 (0- 10² ng/ml). RLU, relative light units. B–L, equal numbers of stably transduced Ba/F3-gp130 cells were cultured for 2 days with increasing amounts of Hyper-IL-6 (0–10² ng/ml) or human IL-6 (0–10² ng/ml). The IL-6R variant is given above the respective diagram. Cellular proliferation was quantified as indicated under “Experimental Procedures.” One representative experiment of two or three performed is shown.