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GGA1 recruitment to M6PR is delayed after BFA washout and co-localizes with TfR. HeLaM cells expressing CD8-M6PR were treated with vehicle (0.175% methanol) or 7.5 μg/ml BFA for 2 min, then BFA was removed, and cells were allowed to recover for the times indicated, fixed, and stained for GGA1 and TfR.
