FIGURE 4.

Analyses of pre-mRNA and splicing. BAL17 cells were induced with either P+I or BCR for the indicated times in minutes. A, time-dependent production of long unspliced RNA was monitored by real-time PCR and is reported relative to β-actin (ActB) expression (normalized to unstimulated cells). The data are reported with a logarithmic y axis. B, association of the RNA splicing factor U2AF65 with c-fos was monitored by ChIP for the indicated times in minutes. Immunoprecipitated DNA was analyzed with primers spanning each of the exon (Ex)/intron (In) borders across the length of the c-fos gene (the exon/intron positions are relative to the TSS depicted in the schematic). The results were analyzed in triplicate and are reported as the mean ± S.D. of the percentage of input DNA. Data are representative of one of three comparable experiments.