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Analyses of egr-2 mRNA and pre-mRNA in primary splenic B cells. Primary B cells were isolated and stimulated as described in the legend to Fig. 5. Isolated total mRNA was analyzed by real-time PCR with primers specific for egr-2 mRNA (A) or pre-mRNA (B). β-Actin (ActB) was used as a normalization control. Ex, exon; In, intron.
