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Effect of c-Rel and RelA silencing on PACAP-stimulated PC12 cell viability. Twenty-four h after transfection with control, c-Rel, or RelA siRNA, PC12 cells were treated by PACAP (100 nm) for 48 h. Cell viability was assessed using MTT assay. Data are expressed as mean ± S.E. (error bars) relative to control values (n = 6). *, p < 0.05 (Mann-Whitney U test). The experiment was repeated two times with similar results.
