TABLE 2.

Kinetic constants of BglA-2 and mutants toward pNPβGlc6P and cellobiose-6′P

Enzyme	pNPβGlc6P			Cellobiose-6′P
	Km	kcat	kcat/Km ×10−1	Km	kcat	kcat/Km ×10−1
	μm	s−1	s−1μm−1	μm	s−1	s−1μm−1
Wild type	478 ± 4.5	195 ± 5.3	4.1 ± 0.3	1135 ± 9.8	170 ± 3.8	1.5 ± 0.2
E171A	NDa	ND	ND	ND	ND	ND
E171Q	ND	ND	ND	ND	ND	ND
E364A	ND	ND	ND	ND	ND	ND
E364Q	ND	ND	ND	ND	ND	ND
Y126A	ND	ND	ND	ND	ND	ND
Y126F	598 ± 3.8	146 ± 6.4	2.4 ± 0.2	1515 ± 11.8	138 ± 7.8	0.9 ± 0.1
Y303A	ND	ND	ND	ND	ND	ND
Y303F	ND	ND	ND	ND	ND	ND
W338A	ND	ND	ND	ND	ND	ND
M423A	512 ± 4.8	168 ± 8.3	3.3 ± 0.4	1320 ± 10.5	145 ± 8.9	1.1 ± 0.3
S424A	ND	ND	ND	ND	ND	ND
K430A	ND	ND	ND	ND	ND	ND
Y432F	ND	ND	ND	ND	ND	ND

^a ND, no detectable activity.