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. 2013 Apr 11;288(21):15006–15014. doi: 10.1074/jbc.M113.458448

FIGURE 3.

FIGURE 3.

Renal fibrosis is aggravated in Dapper3−/− mice after UUO. A, Dapper3 mRNA levels from the kidneys of Sham-operation and UUO mice for 14 days were detected by real-time RT-PCR. B, Masson's trichrome staining of wild-type and Dapper3−/− kidney sections from Sham-operated, 7-day, and 14-day UUO groups is shown. Scale bars, 100 μm. C, relative fibrotic area statistics of Masson's trichrome staining in B is shown. *, p < 0.05. n = 3. D, Picrosirius red staining of kidney sections of wild-type and Dapper3−/−mice after UUO for 7 days is shown. Scale bars, 100 μm. E and F, shown are relative mRNA levels of Col1a1 (E) and Col3a1 (F) from wild-type and Dapper3−/− kidney after Sham-operation or UUO for 14 days. NS, not significant. G, shown is a Western blot of collagen I (col I) protein in contralateral and 14-day UUO samples. GAPDH is used as a loading control. Quantification of collagen I protein levels relative to GAPDH is shown below each band.