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. 2013 Apr 11;288(21):15006–15014. doi: 10.1074/jbc.M113.458448

FIGURE 5.

FIGURE 5.

Enhanced activation of the Wnt/β-catenin canonical pathway in Dapper3−/− obstructive kidneys. A, shown is immunohistochemical staining of β-catenin in wild-type and Dapper3−/− kidneys in different groups: Sham controls, 7 days after UUO, and 14 days after UUO. Two representative areas with β-catenin staining in 14-day UUO group are enlarged in the right boxes. Nuclear localization of β-catenin in tubular epithelial cells (arrowheads) is increased in Dapper3−/− kidneys after UUO than that in wild-type ones. Scale bars, 100 μm. n = 3. B, Western blot analysis shows a further increase in renal β-catenin and Dvl2 levels 7 days after obstructive injury in Dapper3−/− group. Quantification of β-catenin and Dvl2 protein levels relative to GAPDH is shown below each band. n = 4. C, a Western blot of β-catenin in the nuclear extracts from kidney tissues shows increased nuclear β-catenin levels in Dapper3−/− group at day 14 after obstructive injury. Quantification of β-catenin protein levels relative to the nucleus marker lamin B is shown below each band. Tubulin is used as the cytosolic marker. D--F, relative mRNA levels of Axin2 (D), c-Myc (E), and cyclin D1 (F) genes in wild-type and Dapper3−/− mice 14 days after Sham-operation or UUO are shown. **, p < 0.01.