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. 2013 Apr 5;288(21):15046–15056. doi: 10.1074/jbc.M113.466136

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — hnRNP-K overexpression induced malignant transformation of NIH 3T3 cells. hnRNP-K-overexpressing cells when xenografted in nude mice lead to tumor formation (A). B, average volume of hnRNP-K induced tumors from six mice is shown. Overexpression of hnRNP-K in NIH 3T3 cells resulted in lung metastasis (C). D, Western blot indicates a high level of expression of MMP-3 in lung tumor tissue extracts. A high level of expression VEGF (an angiogenesis marker) was detected in hnRNP-K-overexpressing NIH 3T3 cells by Western blotting as well as immunostaining (F). Actin was used as a loading control.