FIGURE 2.

Binding of bA2 WT and variants with FIXa. Biotinylated FPR-FIXa was immobilized on a streptavidin chip and the kinetics with baculovirus-expressed A2 domain (25 nm) were monitored at a flow rate of 20 μl/min for a 3-min association with subunit followed by a 4-min dissociation after return to buffer flow. Representative curves for bA2 forms correspond to (1) WT, (2) K707A, (3) S709A, (4) S710A, and (5) K713A with data represented by the bold line, and fitted curve shown by the dashed line.