FIGURE 6.

Gluconate production is regulated by the noncoding small RNA crcZ. A, schematic regulatory network of Crc in P. aeruginosa. Relevant Crc targets are shown, but this does not represent all possible targets: transporters/permeases (i) and metabolic enzymes (ii). Gluconate is increased for rpoN and cbrA/B mutants, so is predicted to be increased for crcZ and unaffected by crc mutation. B, gluconate levels in mid-exponential phase for PAO1 wild-type, a crcZ mutant, and a crc mutant strain. Gluconate levels were quantified by enzymatic assay (data are mean ± S.E., n = 3) and were significantly different (p < 0.05) between the WT and crcZ deletion strain (Welch's t test).