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pH-dependent transport of Gln by GadC-ΔC is shifted ∼1 pH unit toward a higher pH compared with that of Glu. A, comparison of the substrate accumulation of Glu and Gln. For Glu, GadC-ΔC exhibited no detectable transport activity at pH above 7.0. In contrast, GadC-ΔC could transport Gln even at pH 8.0. B, comparison of Vmax values of GadC-ΔC for Glu and Gln at different pH values.
