TABLE 3.
Kd values for LRP1 ligands binding to WT-LRPIV and LRPIV-D3674G
Values are means ± S.E., n = 3 assays/group.
| Ligands | WT-LRPIV Kd | LRPIV-D3674G Kd | LRPIV-D3674G/WT-LRPIV Kd ratio |
|---|---|---|---|
| nm | nm | ||
| APOE2 | 24.4 ± 1.9 | 40.8 ± 2.6a | 1.6 |
| APOE3 | 29.3 ± 2.1 | 54.3 ± 6.4a | 1.8 |
| APOE4 | 39.5 ± 3.2 | 49.9 ± 5.4a | 1.3 |
| tPA | 106.3 ± 20.7 | 284.5 ± 35.0a | 2.7 |
| MMP-9 | 141.2 ± 25.5 | 575.3 ± 137.3a | 4.1 |
| Factor IXa | 158.3 ± 10.3 | 601.9 ± 40.2a | 3.8 |
a p < 0.05. The difference in Kd values between WT-LRPIV and LRPIV-D3674G was considered to be significant at p < 0.05.