TABLE 1.
Primers for qRT-PCR quantitation of STAMP and TTLLs
Primers for the indicated positions of the listed genes are given in the 5′ to 3′ direction. The primers for STAMP spanning exons 23 and 24/25 detect only the full-length transcripts, whereas the exon 22 and 25 primers amplify the STAMP transcripts of both the wild type and TM mutant mice. Two different primer pairs were used for all TTLL genes except TTLL7.
| Gene | GenBankTM accession number | Region | Forward primer/reverse primer |
|---|---|---|---|
| STAMP | NM_001081423.2 | Exons 23 and 24/25 | GCGCAGGAGAATCCTTGCCCA |
| ACCTCCTCCAGTTCAGCCTCACT | |||
| STAMP | NM_001081423.2 | Exons 23 and 24/25 | CGGTTGGCACTTCTGGAGCG |
| TCCTCCAGTTCAGCCTCACTTGCT | |||
| STAMP | NM_001081423.2 | Exons 22 and 25 | ATGTGCAAATCCGTCTGACA |
| CCTTTTGCCCCACTATCAGA | |||
| TTLL1 | NM_178869.4 | Residues 1359–1479 | GATCGAGGTCAACGCGTCCCC |
| GCAGTCGGGAATCTCGCCGT | |||
| TTLL1 | NM_178869.4 | Residues 1253–1379 | TCGTGCAGTCTCTCAAGGCCG |
| GGGGACGCGTTGACCTCGAT | |||
| TTLL4 | NM_001014974.1 | Residues 2413–2562 | CCTGTCTCGCATGCAAAGCCGT |
| CGAGCAGATGCTGGTGGCTTCA | |||
| TTLL4 | NM_001014974.1 | Residues 3532–3661 | TCTCACCACCCAGTGGGAACAGA |
| TGGCAGAGACCACAGTGGGGC | |||
| TTLL6 | NM_172799.4 | Residues 1890–2010 | TGCCCAGCCCATAACGTCTGT |
| ACTCTCCGGGCTGCTGCACT | |||
| TTLL6 | NM_172799.4 | Residues 1430–1576 | AGCTGTATGCACGGCAGCTGAT |
| GTCTCTGTCGCTGGAGCCGC | |||
| TTLL7 | NM_027594.1 | Residues 1909–1991 | CCAACGTCAGCGTCCCGGTC |
| TGCCGCAAAGACTCGTTCCACT | |||
| TTLL11 | NM_028921.2 | Residues 1418–1487 | TGATCAGAGACACGCTGCGCC |
| TCCATCTGCTGAGACTGGATCTCCT | |||
| TTLL11 | NM_028921.2 | Residues 1384–1487 | CCCAGCCTGGTGGATGAAGAGGT |
| TCCATCTGCTGAGACTGGATCTCCT |