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. 2013 Mar 21;288(21):15256–15267. doi: 10.1074/jbc.M113.461137

FIGURE 2.

FIGURE 2.

Effects of DHS knockdown on T. brucei growth and survival. Panel 1, effects of TbDHSc knockdown; panel 2, effects of TbDHSp knockdown. A and D, cell growth curve of log(cell number × dilution factor) over time. Data represent an average mean ± S.E. for multiple independent biological replicates. A, TbDHSc cDKO cells (n = 6); D, TbDHSp cDKO cells (n = 3); blue circle, + Tet (0.5 μg/ml); green square, −Tet. Panel inset, representative Western analysis performed with rabbit polyclonal antibodies to the indicated protein (30 μg of total protein); TbDHODH was detected as a loading control. B and E, qPCR analysis of mRNA levels for TbDHSc cDKO cells (B) and TbDHSp cDKO cells (E). The symbol < indicates RNA levels were below the limit of detection. Error bars represent the mean ± S.D. for n = 3 replicates. C and F, Kaplan-Meier survival curves of infected mice (n = 3 per group). C, TbDHSc cDKO; F, TbDHSp cDKO cells; SM (single marker); T. brucei wild-type cells (purple), and cDKO-infected mice treated with (blue) or without (green) Dox.