TABLE 2.

UTR sequence of TbDHSc and TbDHSp

5′UTRs for TbDHSc (Tb927.10.2750) and TbDHSp (Tb927.01.870) were cloned by amplification from Tb427 cDNA using the splice leader sequence as a primer with a reverse primer in the ORF. Sequences of the cloned fragments are displayed. The spliced leader sequence is not shown.

5′UTR for Tb927.1.870

5′GCAGTGTCTACAACGCCATAAGGGGGGGGGGTGGTGTGCCGAGCTGTTTGTAGGGCGGCTGGAACGTT TGCAATAGGAGATCGGAAGCAGGAGGGTGATAGCAGTTATAGCCTTTACGGACCATCTTAAGTGAGAAATCCAACTTCAATT CTGCATCCGTTCTTTGAGGTACAAAAAACAAAAAGAGTGCGCACGGTGCCTTGGCAGTTGCTGTCGTGCGGTTC(ATG)

5′UTR for Tb927.1.2750

5′ACTACCGTTTCTTCCCTTCCGGGTCTTGCAACAGCTCGTGCTCTGAGGAAGTGCGTGTTTTGTCTTCA CATCACTTCGTTATTGGGCTTCTTTTCACCAACAACCAACACCACAAATCGTCACACCTCGTCACGCCGTCGCCGTTCTTGT AAATTTTTTTCTTCTGTCTAAGCCACTCAGGTTGGAGTTGTCGATTTTATCGCCAGGGAAAGCAGGATAAGTGGTAAGGGGA TAAAACTAAAAAGCCTCTTATTTGCAACTCCAGACCGCGTGTGAAGTGGAGAATAAGG(ATG)