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Steady-state kinetic parameters for T. brucei heterotetrameric DHS
Variable concentrations of the substrate under determination were used with fixed concentrations (1 mm NAD+, 100 μm TbeIF5A, and 100 μm spermidine) of the other substrates. Error represents the standard deviation for three independent experiments.
| Substrate | Kmapp | kcat |
|---|---|---|
| μm | s−1 | |
| NAD+ | 82 ± 16 | 0.018 ± 0.001 |
| TbeIF5A | 0.7 ± 0.1 | 0.018 ± 0.001 |
| Spermidine | 43 ± 5 | 0.015 ± 0.001 |
