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Early developmental phenotypes of grlB mutants. 5 × 106 vegetative cells were washed with DB twice and plated on non-nutrient DB-agarose plates. Images were taken at indicated hours after development. AX2::grlB, grlB was overexpressed in wild-type AX2 cells; grlB−::grlB-gfp, grlB-gfp fusion was overexpressed in grlB− cells. Bar, 1 mm.
