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. 2013 Apr 2;288(21):15291–15302. doi: 10.1074/jbc.M113.458901

FIGURE 4.

FIGURE 4.

Imaging of primary microglia in Matrigel in vitro. Three-dimensional reconstructions of primary actin-eGFP microglia plated in Matrigel capture the complex, process-bearing morphology of primary microglia. The figure shows an example of a resting, HBSS-treated microglia (control; A) or a microglia activated with 100 ng/ml LPS (B). Treatment with 30 μm NE induced process retraction in both cases. Changes in the surface area-to-volume ratios (SA/V) correlate to changes in ramification. Scale bars, 10 μm.