FIGURE 1.

APAP-induced liver injury in Wt and Cot/tpl2-deficient mice. A, survival of Wt (n = 8) and Cot/tpl2 KO mice (n = 8) after APAP administration (600 g/kg, intraperitoneal). B, Cot/tpl2 KO mice were injected with APAP (450 g/kg, intraperitoneal), and serum ALT and AST levels were measured at various times. Data represent the mean ± S.D. from four experiments performed in quadruplicate. C, Wt and Cot/tpl2 KO mice were injected (450 g/kg, intraperitoneal) with APAP or with PBS for 24 h. H&E staining of liver sections. Images from PBS-injected mice are representative of three mice, and those from mice that received APAP are representative of seven animals from which 7 randomly selected fields were analyzed. Scale bars, 100 μm. Graph shows the mean ± S.E. from the % congestive area analyzed in 49 different fields obtained from 7 APAP-treated Wt, 7 APAP-treated Cot/tpl2 KO mice, and from 3 WT PBS-injected and 3 Cot/tpl2 KO PBS-injected mice. D, Wt and Cot/tpl2 KD mice were injected with APAP (450 g/kg, intraperitoneal), serum ALT and AST levels were determined 18 h later. Graphs represent the mean ± S.D. of three independent experiments performed in quadruplicate.