FIGURE 1.

Relative expression level of the three major Ras isoforms in HAECs and other types of ECs. A, relative expression levels of Ras isoforms in untreated HAECs, HUVECs, cardiac mouse ECs, and lung mouse ECs normalized to β-actin were measured with TaqMan quantitative PCR of mRNA expression levels. n ≥ 3. Error bars, S.E. B, efficacy of H-Ras and N-Ras knockdown. Endogenous H-Ras mRNA was knocked down by targeting the 3′-untranslated region. Relative expression levels of HAECs treated with siControl or siH-Ras are shown. Two-way ANOVA and Bonferroni's post-test were used (n ≥ 4). Error bars, S.E. ***, p < 0.001, siControl versus siH-Ras; n.s., not significant.