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. 2013 Apr 11;288(21):15402–15417. doi: 10.1074/jbc.M113.466789

FIGURE 6.

FIGURE 6.

HDX-MS analysis of peptide YVHATPLIRRLARE (325–338) from 3-lip E2p alone (black squares) versus 3-lip E2p-E3 subcomplex (red circles). A, relative deuterium incorporation as a function of exchange time of 20 s to 1000 min (time in log scale). B, relative peak width analysis by determining peptide isotopic mass envelope width at 20% of the maximal peak height. The error bars for A and B were plotted as the standard deviation of three independent experiments. C, mass spectra of 2+ charged form of peptic fragment YVHATPLIRRLARE (3-lip E2p 325–338). Deuterium uptake of the peptide from 3-lip E2p alone (left) versus 3-lip E2p-E3 subcomplex (right) at 0 s (nondeuterated reference) and 20 s and 1, 3, 10, 30, 100, and 1000 min is shown.