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. 2013 Mar 14;133(2):298–308. doi: 10.1093/toxsci/kft069

Fig. 10.

Fig. 10.

Pyknotic nuclei in different regions of the GD9 embryos exposed to HU. (A) Propidium iodide staining of the RNE, CNE, NT, somite (S), and heart (H) in saline and HU-exposed embryos at 0.5, 3, and 6h posttreatment. The arrowheads indicate pyknotic nuclei. (B) Quantification of pyknotic nuclei by IMARIS. Each bar (mean ± SEM) represents the percentage of pyknotic nuclei. Embryos from five litters per treatment group were analyzed. Asterisks, dagger, and double dagger denote a significant difference from saline control at the same time point (*p < 0.05; †p < 0.01; ‡p < 0.001, Dunnett’s test).