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Real-time quantitative PCR analysis of (A) p53 and bax for apoptosis; (B) pax6a, pax6b, otx2, and rx1 for eye embryogenesis; and (C) sox10, mitfa, and tyrp for pigmentation at the exposure of 0, 1, 10, and 30mg/l TMAT-AuNPs at 96 hpf, respectively. Error bars represent as mean ± SE of at least three replicates. Asterisks represent the significant difference to the control by one-way ANOVA, followed by Tukey’s post hoc test (*p < 0.05).
