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. 2013 May 21;4(3):e00039-13. doi: 10.1128/mBio.00039-13

FIG 1 .

FIG 1 

Complementation of ppk2::Tn. (A) RT-PCR analysis of wild-type M. tuberculosis CDC 1551 expression of intergenic regions from MT3331 to desA3/MT3326 during late log phase. Chromosomal DNA was used as a control. Primer sets 1 to 5 (Table 1) target specific intergenic areas, as indicated in the figure; primer set 6 targets ppk2. (B) PCR analysis of genomic DNA from the wild-type, ppk2::Tn, and ppk2::Tn Comp strains. Primer set A targets the ppk2 gene (bp −50 to 430), yielding a 480-bp product in the wild type, a 2,547-bp product in the ppk2::Tn mutant, and both products in the ppk2::Tn Comp strain. Primer set B targets bp 108 to 661  of ppk2, yielding a 553-bp product in all strains. (C) Southern hybridization demonstrating binding of the ppk2-specific probe to KpnI-digested fragments of the expected size in the wild type (1,669 bp), ppk2::Tn mutant (2,266 bp), and ppk2::Tn Comp strain (2,266 and 6,095 bp). (D) RT-PCR analysis of ppk2 expression in the wild-type, ppk2::Tn, and ppk2::Tn Comp strains during early stationary phase of growth.