TABLE 1 .
Primers used in this study
| Primer | Directiona | Sequence |
|---|---|---|
| Primers used for coexpression studiesb | ||
| Primer set 1 | F | GTAACGAATTCCGGCCAACT |
| R | TACATCTGCGACTGGGTTCC | |
| Primer set 2 | F | CCAAGCTGCTGGAGACCTAC |
| R | CGGGAAGATGGGCAATATAA | |
| Primer set 3 | F | GTGGAAAAGCCAAAGGTCAA |
| R | ACCTGATCGATCGTGATTGG | |
| Primer set 4 | F | ACCAAGAGCTGCCATTTCTG |
| R | CACAATGGTCCCTGGCTTTA | |
| Primer set 5 | F | CGAAGTGGCAAGAGCGTAG |
| R | CCGATCTCCATGTTTTCGAT | |
| Primer set 6 (ppk2) | F | GTGGATATACCATCCGTTGATGTG |
| R | GCGTACAGAAACCCATGACC | |
| sigA primer set | F | ACCTCAAACAGATCGGCAAG |
| R | TGGATTTCCAGCACCTTCTC | |
| Primers used for complemented strain generation/confirmationc | ||
| 1st cloning | F | GCGGAGCTC GTACTCGACGACGGGTGTG |
| R | GCGTCTAGACAACGAGTCGCTGCAACA | |
| 2nd cloning | F | CGTCGCTCATGAGATGGTGCCCTTGGTGG |
| R | GCATCGAACGTTATGTTAAAACGACGGCCAGTGAAT | |
| Primer set A | F | GGTACTCGGTGATCCGTTTG |
| R | AAGCACTGGACGAGCTTCTG | |
| Primer set B | Same as primer set 6 | |
| ppk2 primer set (probe in Southern blot) | F | CGTCTACCAAGCCGAATTGT |
| R | GCACCATCATCTCGTCCTTT | |
| Gene-specific primers used for qRT-PCR studies | ||
| Rv0496 | F | AGAGGACCCTAACGGCAAAT |
| R | TTTCCACCGCTTCTATCGAC | |
| Rv1026 | F | AACTCGATTCGCTTGCTGAT |
| R | TGGTGAAACGTCAGCAGTTC | |
| ppk1 | F | CTCAAGACGCACTGCAAGAC |
| R | TGAACAAGTCGGTCAAGTCG | |
| sigA | F | TCGAGGTGATCAACAAGCTG |
| R | TGGATTTCCAGCACCTTCTC |