FIG 6 .

Viral genomes are maintained in ESCs for at least 10 days. (A) ESCs were mock infected (M) or infected with HCMV strain AD169 (AD) or FIX at an MOI of 3. Total DNA isolated at the indicated day postinfection (dpi) was analyzed by PCR for the presence of viral (UL123) or cellular (GAPDH) DNA. (B and C) Viral genomes at the indicated dpi were quantitated by Li-Cor imaging for three independent biological replicates (B) or by real-time PCR for two biological replicates (C), normalized to cellular genome levels and are expressed as a percentage of the viral genome level detected on day 1 for each individual viral strain.