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5′ RACE analysis of the dmyc cDNA ends detects splice junctions of the transcript.
Notes:dmyc genomic organization (not drawn to scale) with the nucleotides at the exon/intron junctions are shown in green and red. Gel picture shows polymerase chain reaction amplification products of the dmyc cDNA with the gene specific reverse primers GSP-C05 and GSP-C06. For all of the amplification reactions, the Universal Primer Mix (UPM, Clontech) was used as forward primer.
