Figure 7.

Combined action of the downstream promoter element and its upstream binding sites are required for the intron 2 activity. (A) The 40 kb dmyc locus with the predicted regulatory elements¹⁴ and the deletion constructs derived from the intron 2 region are shown. (B–J) Fusion of the enhancer region (J8.4) to the downstream promoter element (J8.5) in J8.3 construct recapitulates the J8/J8.2 transgenes activity in the larval brain and discs (B–E), during embryogenesis (F–I embryo stages: F, 2–5; G, 9–12; H, I, 12–15), and in the ovary (J).

Notes: Staining time for discs and the ovary is indicated above the scale bar; embryos were stained overnight. Scale bar in (B–J) indicates 50 μm.