Table S1.
No. | Name | Primer Sequence (5′→3′) | bp | Usage |
---|---|---|---|---|
1 | BAC-F | CTT CGC GTC CAA CAG ATG | 18 | pC-RP27 sequencing |
2 | BAC-R | GCG CCA AAG CAA GAG GGA ATG | 21 | |
3 | pZpA-F | CCA AAA AAG GGT TTC ATT AAC TTG TAC ACA TAC | 33 | pLacZ-SV40 p(A) sequencing |
4 | pZpA-R | TTG GGG ATT TTA ATA GCG GGC CCT GTG TGT | 30 | |
5 | SV40 | GTT CAG GGG GAG GTG TGG G | 19 | SV40 P(A) sequencing |
6 | pZR | CGG GCC TCT TCG CTA TTA CG | 20 | pJ8 reverse sequencing |
7 | pcaF | GAC GGC GAT ATT TCT GTG GAC | 21 | pCaSpeR4 and |
8 | pcaR | CCT TAG CAT GTC CGT GGG GTT TGA | 24 | pJ8.4 sequencing |
9 | In2E-F-Not | 32 34 |
PCR – pJ8.4 | |
10 | In2E-R-Asp | |||
11 | pDPE-F | 36 | PCR – pJ8.5 | |
12 | pDPE-R | ATG CGC TCA GGT CAA ATT CAG ACG | 24 | |
13 | Intr2-R 01 | CCT CAT CTG CAC AGC GAT AGT A | 22 | pJ8.5 sequencing |
14 | Intr2-R 02 | TCA TCT GCA CAG CGA TAG TAA | 21 | |
15 | Intr2-R 03 | CTG CAC AGC GAT AGT AAA | 18 | |
16 | Intr2-R 04 | GGG GAA GGG GGA GGT GGA GAA GA | 23 | |
17 | GSP-N01 | GCC GGG CGG TAT TAA ATG GAC CTC GTC CTG | 30 | 5′ RACE cDNA synthesis |
18 | GSP-C05 | CTA GCC CTA CGC CGC CGC TTT AAG CCG ATA GTA TGA GAC | 39 | 5′ RACE cDNA synthesis |
19 | GSP-C06 | CAC TGC GCC GCT GCC AAT TAC GAT CCA TG | 29 | |
20 | UPM-sh | CTA ATA CGA CTC ACT ATA GGG C | 22 | 5′ UTR RACE cDNA sequencing |
21 | Exn1-R2 | AAA CAG GCA AAA ATC CAA TCC ACA | 24 | 5′ UTR RACE cDNA sequencing |
22 | Exn1-R3 | GGT TAT TTC TGG GAT TTA GCA CTT | 24 | |
23 | Exn2-F | ACC GGC CAG CAG CAG TCC A | 19 | 5′ RACE cDNA sequencing |
24 | Exn2-R1 | GAA TGG TGG ACG ACG AAA GGA GTT | 24 | |
25 | Exn2-R2 | CTC TGG CTT CAT ATC CTC CTC TAA | 24 |
Notes: The oligonucleotides were designed with Bioinformatics tool Laser Gene 9.1 module Primer Select and synthesized at Microsynth, Switzerland. As indicated the primer sets were used for sequencing and/or amplification of dmyc non-coding regions, all 5′ RACE experiments, and for sequencing of cloning vectors and injection plasmids.
Abbreviations: bp, base pairs; E, exon; F, forward; PCR, polymerase chain reaction; R, reverse; RACE, rapid amplification of cDNA ends; Exn, exon; Intr, intron; DPE, downstream promoter element; UPM-sh, Universal Primer Short.