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. 2013 May 15;7:85–102. doi: 10.4137/GRSB.S10751

Table S1.

Analytical primer pairs.

No. Name Primer Sequence (5′→3′) bp Usage
1 BAC-F CTT CGC GTC CAA CAG ATG 18 pC-RP27 sequencing
2 BAC-R GCG CCA AAG CAA GAG GGA ATG 21
3 pZpA-F CCA AAA AAG GGT TTC ATT AAC TTG TAC ACA TAC 33 pLacZ-SV40 p(A) sequencing
4 pZpA-R TTG GGG ATT TTA ATA GCG GGC CCT GTG TGT 30
5 SV40 GTT CAG GGG GAG GTG TGG G 19 SV40 P(A) sequencing
6 pZR CGG GCC TCT TCG CTA TTA CG 20 pJ8 reverse sequencing
7 pcaF GAC GGC GAT ATT TCT GTG GAC 21 pCaSpeR4 and
8 pcaR CCT TAG CAT GTC CGT GGG GTT TGA 24 pJ8.4 sequencing
9 In2E-F-Not graphic file with name grsb-7-2013-085f9.jpg 32
34
PCR – pJ8.4
10 In2E-R-Asp graphic file with name grsb-7-2013-085f10.jpg
11 pDPE-F graphic file with name grsb-7-2013-085f11.jpg 36 PCR – pJ8.5
12 pDPE-R ATG CGC TCA GGT CAA ATT CAG ACG 24
13 Intr2-R 01 CCT CAT CTG CAC AGC GAT AGT A 22 pJ8.5 sequencing
14 Intr2-R 02 TCA TCT GCA CAG CGA TAG TAA 21
15 Intr2-R 03 CTG CAC AGC GAT AGT AAA 18
16 Intr2-R 04 GGG GAA GGG GGA GGT GGA GAA GA 23
17 GSP-N01 GCC GGG CGG TAT TAA ATG GAC CTC GTC CTG 30 5′ RACE cDNA synthesis
18 GSP-C05 CTA GCC CTA CGC CGC CGC TTT AAG CCG ATA GTA TGA GAC 39 5′ RACE cDNA synthesis
19 GSP-C06 CAC TGC GCC GCT GCC AAT TAC GAT CCA TG 29
20 UPM-sh CTA ATA CGA CTC ACT ATA GGG C 22 5′ UTR RACE cDNA sequencing
21 Exn1-R2 AAA CAG GCA AAA ATC CAA TCC ACA 24 5′ UTR RACE cDNA sequencing
22 Exn1-R3 GGT TAT TTC TGG GAT TTA GCA CTT 24
23 Exn2-F ACC GGC CAG CAG CAG TCC A 19 5′ RACE cDNA sequencing
24 Exn2-R1 GAA TGG TGG ACG ACG AAA GGA GTT 24
25 Exn2-R2 CTC TGG CTT CAT ATC CTC CTC TAA 24

Notes: The oligonucleotides were designed with Bioinformatics tool Laser Gene 9.1 module Primer Select and synthesized at Microsynth, Switzerland. As indicated the primer sets were used for sequencing and/or amplification of dmyc non-coding regions, all 5′ RACE experiments, and for sequencing of cloning vectors and injection plasmids.

Abbreviations: bp, base pairs; E, exon; F, forward; PCR, polymerase chain reaction; R, reverse; RACE, rapid amplification of cDNA ends; Exn, exon; Intr, intron; DPE, downstream promoter element; UPM-sh, Universal Primer Short.