Table 1.
Testicular lesion score (TLS), in situ hybridization (ISH), and apoptosis in boars at different days post-inoculation (dpi)
|
DPI |
n |
PRRSV |
TLS |
Number of positive germ cells for ISH |
Apoptosis |
|||
|---|---|---|---|---|---|---|---|---|
| Spermatogonia | Spermatocyte | Spermatid | Non-sperm cell | |||||
| 14 |
3 |
Type 1 |
5.9 ± 1.5 |
1.4 ± 0.8 |
2.5 ± 0.6 |
2.0 ± 0.6 |
1.4 ± 0.4 |
35.2 ± 1.4b |
| |
|
Type 2 |
6.0 ± 1.4 |
1.1 ± 0.6 |
2.1 ± 0.4a |
1.9 ± 0.7 |
1.4 ± 0.4 |
28.4 ± 5.8 |
| 21 |
3 |
Type 1 |
7.1 ± 1.0 |
0.9 ± 0.5 |
1.9 ± 0.5a |
1.1 ± 0.7 |
1.2 ± 0.3 |
23.6 ± 7.2 |
| |
|
Type 2 |
7.2 ± 1.2 |
0.9 ± 0.5 |
1.7 ± 0.4 |
1.3 ± 0.4 |
1.1 ± 0.7 |
21.6 ± 6.8 |
| 35 |
3 |
Type 1 |
8.5 ± 0.5 |
0.5 ± 0.7 |
0.9 ± 0.5 |
0.5 ± 0.5 |
0.3 ± 0.4 |
16.0 ± 4.2 |
| Type 2 | 8.4 ± 0.6 | 0.4 ± 0.6 | 0.8 ± 0.4 | 0.6 ± 0.4 | 0.4 ± 0.5 | 16.2 ± 6.4 | ||
aSignificantly different PRRSV-positive cells between spermatogonia and spermatocyte.
bSignificantly different numbers of apoptosis between type 1 and type 2 PRRSV-infected boars.