Table 1.
Example PA-FP on-state properties
| PA-FP classes | ex (nm) | em (nm) | Brightnessa | Oligomeric state | Contrast | Reference |
|---|---|---|---|---|---|---|
| Irreversible, off to on | ||||||
| PAGFP | 504 | 517 | 13750 | monomer | 70 | [5] |
| PAmCherry1 | 564 | 595 | 8280 | monomer | 4000 | [7] |
| Photoconversion, wavelength shift | ||||||
| PS-CFP2 | 490 | 511 | 10810 | monomer | >2000b | [21] |
| Kaede | 572 | 582 | 19900 | tetramer | 2000c | [11] |
| KikGR | 583 | 593 | 21200 | tetramer | >2000c | [12] |
| mKikGR | 580 | 591 | 17650 | tetramer | NR | |
| Dendra2 | 553 | 573 | 19250 | monomer | 300 | [14] |
| EosFP | 571 | 581 | 22600 | tetramer | NR | [13] |
| mEos2 | 573 | 584 | 30300 | monomer | NR | [19] |
| Reversible photoactivation | ||||||
| Dronpa | 503 | 518 | 80800 | monomer | NR | [30] |
| rsFastlime | 496 | 518 | 30100 | monomer | 70d | [23] |
| Padron | 396 | 522 | 27500 | monomer | 140d | [24] |
| KFP1 | 580 | 600 | 4100 | tetramer | >30 | [26] |
| rsCherry | 572 | 610 | 1600 | monomer | 7d | [25] |
| rsCherryRev | 572 | 608 | 420 | monomer | 20d | [25] |
NR, not reported.
Product of extinction coefficient and quantum yield.
Determined by the green-to-cyan fluorescence ratio after photoactivation divided by the green-to-cyan fluorescence ratio before photoactivation.
Determined by the red-to-green fluorescence ratio after photoactivation divided by the red-to-green fluorescence ratio before photoactivation.
Determined by ratio of the maximum signal divided by the residual off-state fluorescence.