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. 2013 May 4;20(1):27. doi: 10.1186/1423-0127-20-27

Figure 1.

Figure 1

Differential recognition of proteins due to methylation status by anti-Sm autosera. HeLa cell extracts (30 μg of total protein) were prepared from cells grown in the presence or absence of 20 μM of AdOx. The cell extracts were separated by SDS-PAGE and transferred onto nitrocellulose membrane. Western blot analysis was performed using Sm1, Sm2 and Sm3 autosera. The positions of the differentially detected signals are indicated by arrows and the intensity ratios are shown. The positions of typical anti-Sm recognized SmB/B′, SmD1 and D3 proteins are also indicated.