Table 1.
Putative differentially recognized polypeptides by the Sm1 serum determined by mass spectrometry
| Spot number | Protein name/gene name | Accession number | Measured MW/pI | Theoretical MW/pI | Mascot score (PMF/MS/MS)* | Arginine methylation sites (predicted or experimental) |
|---|---|---|---|---|---|---|
| 1 |
Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial, OXCT1 |
P55809 |
53/7.8 |
56578/7.14 |
64/173 |
None |
| Aldehyde dehydrogenase X, mitochondrial, ALDH1B1 |
P30837; B2R8F0; Q8WX76; Q9BV45 |
57626/6.36 |
88/34 |
None |
||
| 2 |
Alpha-enolase, ENO1 |
GMP2; Q71V37; Q7Z3V6; Q8WU71; Q9UCH6; Q9UM55 |
40/7.7 |
47481/7.01 |
x /97 |
None |
| Splicing factor, proline- and glutamine-rich, SFPQ |
P23246; P30808; Q5SZ71 |
76216/4.95 |
x/52 |
RFRSRGGGGGFHRRG GGGGRGG (7, 9, 19, 25;) [19]; EEERRRRE (571); RGMGPGTPAGYGRG (681,693) [20] |
||
| 3 |
Putative beta-actin-like protein 3, POTEKP |
Q9BYX7; Q562N5 |
40/8.1 |
41989/5.91 |
x/95 |
None |
| Alpha-enolase, ENO1 |
|
47481/7.01 |
x/84 |
None |
||
| 4 |
Alpha-enolase, ENO1 |
|
39/8.3 |
47481/7.01 |
99/202 |
None |
| 5 |
LIM and SH3 domain protein 1, LASP1 |
Q14847; Q96ED2; Q96IG0 |
33/7.8 |
30097/6.61 |
(50)/149 |
None |
| 6 |
Heterogeneous nuclear ribonucleoprotein D-like, HNRNPDL |
O14979; Q6SPF2; Q7KZ74; Q7KZ75; Q96IM0; Q96S43 |
34/8.3 |
46580/9.59 |
none/55 |
STYGKASRGG GNHQ (408) [19] |
| 7 | Cellular nucleic acid-binding protein, CNBP | Q5U0E9; Q6PJI7; Q96NV3 | 19/7.9 | 20704/8.00 | x/56 | TGGGRGRGMR SRGRGGFTSD RGFQFVSSSL [20] |
*x indicates that the PMF search results were not consistent with the MS/MS results. “None” indicates that none of the PMF (peptide mass fingerprint) results (protein scores) are significant. The protein score in parentheses is not significant.