Figure 5. Proteomic analysis.

Representative image from three independent experiments of Coomassie-stained 2-DE patterns of DAOY(A), UW228 (B) and ONS-76 (C) cell lines. Proteins were separated using 7-cm, pH-3-10 NL strips followed by SDSPAGE on 10%, 7×10-cm gels. Proteins showing differential expression were indicated with different colors: in blue –proteins identified in all cell lines and listed in Table 2; in black-proteins identified in adherent cells and spheres of each cell lines and in red-proteins only identified in spheres. Corresponding identifications are reported in Table 3, 4 and 5.